GSM5261561: MCF7-3xFlag-KI-CTR9: Flag-CST-Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTR9
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7-3xFlag-KI-CTR9
cell line
MCF7
cell type
human luminal breast cancer
genotype/variation
3xFlag Knock in at TSS of CTR9 gene
chip antibody
Anti-DYKDDDDK Tag (Cell signaling technology, Cat# 14793, RRID:AB_2572291)
treatment
N/A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with corresponding antibodies. Libraries were prepared according to manufacturer's protocol of the Ovation Ultralow System V2 1-16 Kit (NuGEN Technologies). Briefly, the DNA was end-repaired and ligated to Illumina sequencing adaptors. The ligated DNA was purified using Agencourt RNAClean XP beads (Beckman Coulter). A subsequent PCR amplification step (8-15 cycles) was performed to add linker sequence to the purified fragments for annealing to the Genome Analyzer flow-cell. Following PCR amplification, the library was separated on a 2% agarose gel (120 V, 1.5 hours) to select a narrow range of fragment sizes, and bands between 200-500 bp were excised. The library was purified from the excised agarose gel using the Qiagen MiniElute PCR Purification Kit following the manufacturer's protocol.